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The MKKK62-MKK3-MAPK7/14 module negatively regulates seed dormancy in rice

发布时间:2019/3/29来源:太阳集团游戏官方网址
Xingxue Mao1,2†, Jianjun Zhang3†, Wuge Liu1,2†, Shijuan Yan4, Qing Liu1,2, Hua Fu1,2, Junliang Zhao1,2, Wenjie Huang4, Jingfang Dong1,2, Shaohong Zhang1,2, Tifeng Yang1,2, Wu Yang1,2, Bin Liu1,2* and Feng Wang1,2*


Rice


Abstract


Background:Seed dormancy directly affects the phenotype of pre-harvest sprouting, and ultimately affects the quality and yield of rice seeds. Although many genes controlling seed dormancy have been cloned from cereals, the regulatory mechanisms controlling this process are complex, and much remains unknown. The MAPK cascade is involved in many signal transduction pathways. Recently, MKK3 has been reported to be involved in the regulation of seed dormancy, but its mechanism of action is unclear.

Results:We found that MKKK62-overexpressing rice lines (OE) lost seed dormancy. Further analyses showed that the abscisic acid (ABA) sensitivity of OE lines was decreased. In yeast two-hybrid experiments, MKKK62 interacted with MKK3, and MKK3 interacted with MAPK7 and MAPK14. Knock-out experiments confirmed that MKK3, MAPK7, and MAPK14 were involved in the regulation of seed dormancy. The OE lines showed decreased transcript levels of OsMFT, a homolog of a gene that controls seed dormancy in wheat. The up-regulation of OsMFT in MKK3-knockout lines (OE/mkk3) and MAPK7/14-knockout lines (OE/mapk7/mapk14) indicated that the MKKK62-MKK3-MAPK7/MAPK14 system controlled seed dormancy by regulating the transcription of OsMFT.

Conclusion:Our results showed that MKKK62 negatively controls seed dormancy in rice, and that during the germination stage and the late stage of seed maturation, ABA sensitivity and OsMFT transcription are negatively controlled byMKKK62. Our results have clarified the entire MAPK cascade controlling seed dormancy in rice. Together, these results indicate that protein modification by phosphorylation plays a key role in controlling seed dormancy.

Keywords:Rice (Oryza sativa L.), Pre-harvest sprouting, Dormancy, MAPK cascade


附件:The MKKK62-MKK3-MAPK7/14 module negatively regulates seed dormancy in rice

































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